Projects implemented in the Laboratory of Molecular Assays and Imaging:

 

Ongoing projects:

  • Tasks carried out as part of EU-OPENSCREEN-ERIC:
    • Screening of the EU-OPENSCREEN Diversity and Bioactives chemical library to select redox active compounds in three reactive oxygen species assays
    • Screening of the EU-OPENSCREEN Diversity and Bioactives chemical library to investigate the interference of test compounds with bioluminescent reporters.
  • (POL-OPENSCREEN) Project of the Polish Platform of Screening Infrastructure for Biological Chemistry, which is part of the EU-OPENSCREEN (European Infrastructure of Open Screening Platforms for Chemical Biology European Research Infrastructure Consortium). Financed by the Ministry of Science and Higher Education.
  • Development of the staining and imaging methodology to monitor the transfer of the Dicer protein from the cytoplasm to the cell nucleus, and the development of a test enabling the assessment of the cytotoxicity mechanism of the tested compounds for the screening in cell painting – in project OPUS 2021/41/B/NZ2/03781 dr hab. Anna Kurzyńska-Kokorniak.
  • Cell-based high-throughput screening for small-molecule compounds diminishing molecular markers of pathogenesis in myotonic dystrophy type 2 (DM2), dr hab. Marzena Wojciechowska.

Completed projects:

  • „High throughput screening of small molecules binding to influenza and coronavirus vRNA”,  – Prof. Elżbieta Kierzek, Department of RNA Structural Genomics, IBCH.
  • “ECBL-based screening of S-adenosyl-L-homocysteine hydrolase from Pseudomonasaeruginosa – towards a new class of inhibitors targeting the enzyme dynamics”- Prof. Krzysztof Brzeziński, Department of Structural Biology of Prokaryotic Organisms, IBCH.
  • EU-OPENSCREEN-DRIVE: Ensuring long-term sustainability of excellence in chemical biology within Europe and beyond. H2020 – H2020-INFRADEV-2018-2020.
  • Development of a cell assays based on dual-analyte probes for the detection of the activity of two human proteases involved in SARS-CoV-2 infection and fluorescence studies of the library of hemicyanin dye derivatives and their cytotoxicity on cell lines in the HOMING POIR.04.04.00-00-441F/17-00 project (Homing/2017-4/33), dr Jacek Kolanowski.
  • In vitro characterization of new bioluminescent probes in the SONATA 2017/26/D/NZ1/01234 project, dr Jacek Kolanowski.
  • FLIM imaging of erythrocytes to investigate the impact of a potential fluorescent probe sensitive to redox reactions – in collaboration with prof. Marek Sikorski, Faculty of Chemistry, Adam Mickiewicz University.
  • Study of the effect of the natural extracts on cancer cells – service for Raytech Sp. z o.o.