Projects implemented in the Laboratory of Molecular Assays and Imaging:
Ongoingprojects:
Tasks carried out as part of EU-OPENSCREEN-ERIC:
Screening of the EU-OPENSCREEN Diversity and Bioactives chemical library to select redox active compounds in three reactive oxygen species assays
Screening of the EU-OPENSCREEN Diversity and Bioactives chemical library to investigate the interference of test compounds with bioluminescent reporters.
(POL-OPENSCREEN) Project of the Polish Platform of Screening Infrastructure for Biological Chemistry, which is part of the EU-OPENSCREEN (European Infrastructure of Open Screening Platforms for Chemical Biology European Research Infrastructure Consortium). Financed by the Ministry of Science and Higher Education.
Development of the staining and imaging methodology to monitor the transfer of the Dicer protein from the cytoplasm to the cell nucleus, and the development of a test enabling the assessment of the cytotoxicity mechanism of the tested compounds for the screening in cell painting – in project OPUS 2021/41/B/NZ2/03781 dr hab. Anna Kurzyńska-Kokorniak.
Cell-based high-throughput screening for small-molecule compounds diminishing molecular markers of pathogenesis in myotonic dystrophy type 2 (DM2), dr hab. Marzena Wojciechowska.
Completedprojects:
„High throughput screening of small molecules binding to influenza and coronavirus vRNA”, – Prof. Elżbieta Kierzek, Department of RNA Structural Genomics, IBCH.
“ECBL-based screening of S-adenosyl-L-homocysteine hydrolase from Pseudomonasaeruginosa – towards a new class of inhibitors targeting the enzyme dynamics”- Prof. Krzysztof Brzeziński, Department of Structural Biology of Prokaryotic Organisms, IBCH.
EU-OPENSCREEN-DRIVE: Ensuring long-term sustainability of excellence in chemical biology within Europe and beyond. H2020 – H2020-INFRADEV-2018-2020.
Development of a cell assays based on dual-analyte probes for the detection of the activity of two human proteases involved in SARS-CoV-2 infection and fluorescence studies of the library of hemicyanin dye derivatives and their cytotoxicity on cell lines in the HOMING POIR.04.04.00-00-441F/17-00 project (Homing/2017-4/33), dr Jacek Kolanowski.
In vitro characterization of new bioluminescent probes in the SONATA 2017/26/D/NZ1/01234 project, dr Jacek Kolanowski.
FLIM imaging of erythrocytes to investigate the impact of a potential fluorescent probe sensitive to redox reactions – in collaboration with prof. Marek Sikorski, Faculty of Chemistry, Adam Mickiewicz University.
Study of the effect of the natural extracts on cancer cells – service for Raytech Sp. z o.o.
