High-throughput screening

  • Screening Assay:
    • Development (including multiparametric)
    • Miniaturisation and optimisation
    • Automation
    • Orthogonal assay

 

  • High Throughput Screening:
    • Assay transfer and automation
    • Primary screening at single concentration
    • Hit validation: 3 concentrations
    • IC/EC50 determination
    • Counter screening
    • Orthogonal screening

 

  • Types of assays we perform in our Centre:
    • Biochemical assays:
      • Absorbance
      • Fluorescence and derivatives
      • Luminescence

 

    • Cell based assays:
      • Target based (ex. Reporter assay)
      • Phenotypic (ex. Cytotoxicity)
      • High Content Screening – “Cell Painting” assay

 

  • Format: 96- or 384-well plates

 

  • Examples of screening assays:
    • Cell Painting
    • Bioprofiling